• PCR and NGS primers for sgRNA/barcode amplification and sequencing (IDT): See NGS Cassette Diagram, provided as a link or attachment in email upon shipment of product

For your convenience, we also offer Library NGS Prep Kits which provide primers and reagents required for amplification and multiplexing of up to 12 samples, as well as custom sequencing primers.

  • Titanium Taq DNA Polymerase with PCR buffer (Clontech-Takara, Cat.# 639242)
  • dNTP Mix (10 mM each) (Thermo Fisher, Cat.# 10297018)
  • QIAquick PCR Purification Kit (QIAGEN, Cat.# 28106)
  • QIAquick Gel Extraction Kit (QIAGEN, Cat.# 28706)
  • PhiX Control (Illumina, Cat.# FC-110-3001)
  • NGS Reagent Kits (Illumina):

    Platform Kit Type Illumina Cat.# Description
    NextSeq®
    Single Read (SR)
    or Paired End (PE)
    Sequencing
    & Cluster Gen.
    20024906 NextSeq 500 v2.5 Kit
    (75 cycles, High-Output)
    HiSeq® 2000/2500
    Single Read (SR)
    Sequencing GD-401-3001 TruSeq SBS Kit v3 – HS (50 cycles)
    Cluster Gen. FC-401-3002 TruSeq SR Cluster Kit v3-cBot-HS
    HiSeq® 2000/2500
    Paired End (PE)
    Sequencing GD-401-3001 TruSeq SBS Kit v3 – HS (50 cycles)
    Cluster Gen. PE-401-3001 TruSeq PE Cluster Kit v3-cBot-HS
    HiSeq® 3000/4000
    Single Read (SR)
    Sequencing FC-410-1001 HiSeq 3000/4000 SBS Kit (50 cycles)
    Cluster Gen. GD-410-1001 HiSeq 3000/4000 SR Cluster Kit
    HiSeq® 3000/4000
    Paired End (PE)
    Sequencing FC-410-1001 HiSeq 3000/4000 SBS Kit (50 cycles)
    Cluster Gen. PE-410-1001 HiSeq 3000/4000 PE Cluster Kit

NOTE: See Illumina website for information on HiSeq 2500 Rapid Run kits. Currently, we DO NOT support NGS on the MiSeq*.

Other than the specific reagents and instruments listed above, the protocols assume the user has access to standard materials (e.g., polypropylene tubes, pipette tips), equipment (table top centrifuges, pipettes, scales), and common reagents (e.g., TE buffer, ethanol) and buffers used in a typical life science laboratory.

Last modified: May 15, 2019

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