Cellecta’s CloneTracker XP™ Lentiviral Barcode Libraries are pooled expressed barcode libraries that enable the tracking and profiling of individual clones derived from a population of cells using either PCR/NGS of barcodes integrated in genomic DNA or RNA-Seq of transcribed barcodes. Pre-made pooled libraries consisting of either 1 million or 10 million barcoded constructs are available, with each construct expressing a unique barcode sequence.
When a cell population is transduced with a CloneTracker XP Barcode Library, the barcodes integrate into the genomic DNA of the cells. The result is a starter founder population where almost every cell has a different barcode integrated into its DNA. Since the barcode is stably integrated, it is passed onto any cell progeny when genomic DNA is replicated. This feature enables identification of all the progeny derived from each individual cell.
When used in conjunction with single-cell RNA-Seq, the expressed barcode may be used to identify expression profiles and activated genes in different cells so that distinct clonal populations of cells from a single progenitor, as well as sub-populations of cells with distinct pathway activations or expression profiles, can be readily identified.
Additionally, CloneTracker XP barcode libraries, similarly to the standard CloneTracker (CellTracker) 50M Barcode Library, allow researchers to track cell proliferation over the course of an experiment. For instance, it is possible to:
- Track the fate of cell clones over a course of drug treatment.
- Study the effect of differentiation on hematopoietic cells.
- Assess the effect of treatment on implanted tumor cell populations.
The CloneTracker XP Barcode Libraries are constructed in Cellecta third-generation lentiviral vectors. The specially-designed, optimized barcodes facilitate Next-Generation Sequencing (NGS) data analysis and barcode identification. Using an Illumina NextSeq, HiSeq, NovaSeq, or MiSeq NGS instrument, barcode sequences are identified and converted to lists with enumerated data.
The protocols included provide guidelines for packaging the CloneTracker XP Barcode Library into VSV-G pseudotyped lentiviral particles, transduction of target cells, and the preparation of barcodes derived from transduced cells for NGS, enumeration, and analysis.
References and Product Citations for all Cellecta products can be found on the Cellecta website:
Please read the entire user manual before proceeding with your experiment. Also, please note that, when working with pseudoviral particles, you should follow the recommended guidelines for working with Biosafety Level 2 (BSL-2) materials.
The purchase of all Cellecta Products is covered by Cellecta’s standard Terms and Conditions of Sale as described on Cellecta’s website, and selected Products containing particular technology or having certain features are also subject to restrictions on use as outlined in the Label License section of our website. Please review these Terms and Label License Restrictions before opening and using your Product and, if you are not able to abide by the restriction, contact Cellecta to return the item to Cellecta for a full refund.
Need more help with this?