The LentiTrans™ Transduction Reagent used during the transduction of target cells with lentivirus contains Polybrene, a transduction enhancement reagent. Some cell lines may be sensitive to this reagent. Before doing large-scale transduction, we recommend checking the toxicity of the LentiTrans Reagent using the procedure below:
- In six wells of a 12-well plate, grow cells for 24 hours in complete culture medium without the LentiTrans Reagent, and with LentiTrans added at 0.25 μl/ml, 0.5 μl/ml and 1:1000 (1 μl/ml).
- Replace old medium with LentiTrans-free complete culture medium and grow cells for an additional 72 hours.
- Check for toxicity by counting viable cells.
For your experiments, use the highest concentration of LentiTrans Reagent that results in less than 10% cell toxicity compared to the no-LentiTrans culture (typically, a 1:1000 is recommended). For a few cell types, you may not be able to use LentiTrans Reagent.
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