The CRISPRaTest™ Functional dCas9-Activator Assay Kit (Cat.# CRATEST) is designed to measure the activity of dCas9 transactivator (e.g., dCas-VPH, dCas9-VPR) in any mammalian cell system. The CRISPR gene activation system (CRISPRa), a modification of the original CRISPR knockout system, uses an sgRNA molecule to direct a catalytic inactive Cas9 (dCas9) fused with a Transcriptional Activator (TA), to a target location on a gene promoter. The sgRNA-dCas9-TA complex then increases the expression levels of the gene transcribed from the targeted the promoter.
The CRISPRaTest Kit provides reagents to measure the activator strength of any Streptococcus pyogenes dCas9 transactivator (dCas9-TA) hybrid protein in cells designed to be used for CRISPRa experiments, using a simple FACS-based assay. The kit contains pseudoviral packaged lentiviral constructs with a green fluorescent protein (GFP) gene that increases in expression when expressed in cells with active dCas9-TA. The kit provides enough reagents to measure this activity in 5-15 cell lines.
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Please read the entire user manual before proceeding with your experiment. Also, please note that, when working with pseudoviral particles, you should follow the recommended guidelines for working with Biosafety Level 2 (BSL-2) materials.
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