The CRISPRiTest™ Functional dCas9-Repressor Assay Kit allows you to measure a functional dCas9-Repressor (e.g., dCas9-KRAB) in most mammalian cell systems. The choice of kit CRISPRiTest™ or CRISPRiTest™ (EF1L), depends on the cell line to be tested. In iPS cells, where the CMV promoter used in the CRISPRuTest™ Kit is quickly silenced, the CRISPRuTest™ Kit (EF1L) is strongly recommended.

The CRISPR interference (CRISPRi) system is a modification of the original CRISPR system that uses a targeted sgRNA molecule to direct a “catalytically dead” Cas9 (dCas9) fused with a gene repressor, for example, KRAB (i.e., dCas9-KRAB), to a target a gene promoter on genomic DNA. The recruitment of the Cas9-Repressor to the promoter region then blocks the expression of the gene regulated by the promoter. The CRISPRiTest Kit provides reagents to measure the repressor efficiency of any Streptococcus pyogenes dCas9 repressor (dCas9-Rep) hybrid protein in cells designed to be used for CRISPRi experiments, using a FACS-based assay. The Kit contains packaged lentiviral constructs expressing dCas9-Rep regulated GFP and a non-regulated RFP protein. The Kit provides enough reagents for 5-10 cell lines.

References and Product Citations for all Cellecta products can be found on the Cellecta website:

Please read the entire user manual before proceeding with your experiment. Also, please note that, when working with pseudoviral particles, you should follow the recommended guidelines for working with Biosafety Level 2 (BSL-2) materials.

PLEASE NOTE: The purchase of all Cellecta Products is covered by Cellecta’s standard Terms and Conditions of Sale as described on Cellecta’s website, and selected Products containing particular technology or having certain features are also subject to restrictions on use as outlined in the Label License section of our website. Please review these Terms and Label License Restrictions before opening and using your Product and, if you are not able to abide by the restriction, contact Cellecta to return the item to Cellecta for a full refund.

Last modified: 17 May 2024

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