Transcriptional repression efficiency of in the dCas9-Rep cell line is calculated as the ratio between the normalized GFP intensity of Parental Cells and the normalized GFP intensity of dCas9-Rep Cells.
The data above was calculated using the suggested positive control MDA-MB-231-dCas9-KRAB and parental MDA-MB-231 cells (see Additional Required Materials). The level of repression is calculated as follows using this data as an example:
|Parental||334||291||334 / 291 = 1.148|
|dCas9-KRAB||27||221||27 / 221 = 0.122|
dCas9-Rep fold repression = 1.148 / 0.122 = 9.4 fold
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