Transcriptional repression efficiency of in the dCas9-Rep cell line is calculated as the ratio between the normalized GFP intensity of Parental Cells and the normalized GFP intensity of dCas9-Rep Cells.

The data above was calculated using the suggested positive control MDA-MB-231-dCas9-KRAB and parental MDA-MB-231 cells (see Additional Required Materials). The level of repression is calculated as follows using this data as an example:

Mean_GFP Mean_RFP GFP/RFP
Parental 334 291 334 / 291 = 1.148
dCas9-KRAB 27 221 27 / 221 = 0.122

dCas9-Rep fold repression = 1.148 / 0.122 = 9.4 fold

Last modified: Jan 10, 2019

Need more help with this?
Contact Us

Thanks for your feedback.