4. Protocol Overview

The CRISPRiTest™ Virus (CiT Viral Mix) contains two premixed lentivectors: (1) a vector expressing GFP from the CMV promoter and a U6-driven sgRNA targeting the CMV-GFP transcription start site and (2) a vector expressing RFP from the CMV promoter and a U6-driven non-targeting sgRNA.

Transduction of CiT Viral Mix into parental cells will result in a mixed population of transduced cells expressing high levels of RFP and high levels of GFP. Transduction of CiT virus mix into dCas9-KRAB cells will result in a mixed population of transduced cells expressing high levels of RFP and low levels of GFP.