The CRISPRuTest™ Functional Cas9 Activity Assay Kit for Any Cell (Mammalian) (Cat. #CRUTEST) can be used to measure the Cas9 nuclease activity in any mammalian cell system. The CRISPR system uses a target single-guide RNA molecule (sgRNA) to target and cut a specific sequence on the genomic DNA with the Cas9 nuclease. The CRISPRuTest Kit provides a FACS-based assay to assess the Cas9 nuclease activity in cells expressing Streptococcus pyogenes Cas9. The kit contains pseudoviral packaged lentiviral constructs with a green fluorescent protein (GFP) gene whose fluorescence is disrupted in cells with active Cas9. The kit provides enough reagents to measure this activity in 5-15 cell lines.
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Please read the entire user manual before proceeding with your experiment. Also, please note that, when working with pseudoviral particles, you should follow the recommended guidelines for working with Biosafety Level 2 (BSL-2) materials.
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