The CRISPRuTest™ Functional Cas9 Activity Assay Kit can be used to measure the Cas9 nuclease activity in most mammalian cell systems. The choice of kit (CRISPRuTest™ or CRISPRuTest™ (EF1L)), depends on the cell line to be tested. In iPS cells, where the CMV promoter used in the CRISPRuTest™ Kit is quickly silenced, the CRISPRuTest™ Kit (EF1L) is strongly recommended.

The CRISPR system uses a target single-guide RNA molecule (sgRNA) to target and cut a specific sequence on the genomic DNA with the Cas9 nuclease. The CRISPRuTest Kit provides a FACS-based assay to assess the Cas9 nuclease activity in cells expressing Streptococcus pyogenes Cas9(spCas9) or Staphylococcus aureus_Cas9 (saCas9). The kit contains pseudoviral packaged lentiviral constructs with a green fluorescent protein (GFP) gene whose fluorescence is disrupted in cells with active Cas9. The kit provides enough reagents to measure this activity in 5-15 cell lines.

References and Product Citations for all Cellecta products can be found on the Cellecta website:

Please read the entire user manual before proceeding with your experiment. Also, please note that, when working with pseudoviral particles, you should follow the recommended guidelines for working with Biosafety Level 2 (BSL-2) materials.

PLEASE NOTE: The purchase of all Cellecta Products is covered by Cellecta’s standard Terms and Conditions of Sale as described on Cellecta’s website, and selected Products containing particular technology or having certain features are also subject to restrictions on use as outlined in the Label License section of our website. Please review these Terms and Label License Restrictions before opening and using your Product and, if you are not able to abide by the restriction, contact Cellecta to return the item to Cellecta for a full refund.

Last modified: 21 March 2024

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