DriverMap Targeted Expression Profiling Kits contain enough reagents for the preparation of NGS samples starting from total RNA from blood, tissue, cells, or other biological samples, including Positive Control RNA.

Box 1: Kit Components

Component Description Cap Color Conc. Volume
(24 rxn)*
Volume
(96 rxn)
Positive Control RNA Total RNA from multiple cell lines, 50 ng/µl white 10X 5 µl 5 µl
Random Primers Random primer mix for cDNA synthesis, 50 µM yellow 10X 30 µl 120 µl
PCR-Grade Water RNase/DNase-free water for reaction mixes white NA 3 ml 12 ml
dNTP Mix Mix of dATP, dGTP, dCTP and dTTP, 10 mM each white 20X – 50X 95 µl 375 µl
RT-EXT Buffer cDNA synthesis and Gene-Specific primer extension reaction buffer green 5X 200 µl 780 µl
Reverse Transcriptase Multiplex-optimized M-MLV Reverse Transcriptase, 200 U/µl green 20X 15 µl 55 µl
PCR Buffer Reaction Buffer for 1st and 2nd PCR steps (note: tube cap says “DNA) red 5X 650 µl 2,500 µl
DNA Polymerase Multiplex-optimized thermostable DNA polymerase, 2 U/µl red 100X 45 µl 165 µl
Forward GS Primer Pool** Mix of Forward Gene-Specific Primers highlight yellow 10X 60 µl 225 µl
Reverse GS Primer Pool** Mix of Reverse Gene-Specific Primers highlight yellow 10X 85 µl 330 µl
Primer Removal Enzyme Enzyme Mix for primer removal orange 150X 15 µl 55 µl
Anchor Primer Mix Forward/Reverse Primers for 1st PCR, 2.5 mM each yellow 10X 280 µl 1,050 µl
Forward SeqDNA NGS Primer Forward NGS Primer for cDNA sequencing blue 100 µM 30 µl 90 µl
Reverse SeqDNA NGS Primer Reverse NGS Primer for cDNA sequencing blue 100 µM 30 µl 90 µl
Forward SeqIND NGS Primer Forward NGS Primer for Index sequencing blue 100 µM 30 µl 90 µl
Reverse SeqIND NGS Primer Reverse NGS Primer for Index sequencing blue 100 µM 30 µl 90 µl

*The 24 reaction Assay Kit is only available for the DriverMap Human Genome-Wide Assay.

**The Forward and Reverse GS Primer Pools are specific for each kit. Please see the Product Insert for the Kit to identify which components correspond to the Primer Pools.


Box 2: Kit Components

Component Description Conc. Volume
(24 reactions)*
Volume
(96 reactions)
Forward & Reverse Index Primer Set Forward/Reverse Primer Pairs for PCR with Indexed Primers, pre-aliquoted in a 96-well plate 10X 5 µl each
(24 wells)*
5 µl each
(96 wells)

*The 24 reaction Assay Kit is only available for the DriverMap Human Genome-Wide Assay.

Kit contents should be stored at -20°C. Shelf life is 6 months.

Last modified: Nov 12, 2019

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