Background
Two-thirds of the human genome is made up of mobile elements comprising Long Interspersed Nuclear Elements (LINEs, such as LINE-1). Members of the L1H subfamily of LINE-1 are active transposons that induce genetic rearrangements throughout the genome. These transpositions contribute to genetic diversity in tumor cell populations and have also been implicated in schizophrenia, Alzheimer’s, and other neurodegenerative diseases. Cellecta’s LINE-1 Mobile Element NGS Assay provides a convenient, efficient, and targeted NGS-based approach to identify de novo polymorphic insertions of LINE-1 in the human genome. The assay uses specially-designed primers to selectively amplify only the transposable elements of the youngest L1H family of LINE-1 that are still active in the genome, as opposed to the ancient elements fixed in the population. The amplified fragments are then characterized by NGS to assess the prevalence and diversity in the genome. This approach enables detection and identification of all the 3’ L1H variations for a sample in a single reaction, enabling rapid analysis of multiple samples.1
[1] Streva, V. A., Jordan, V. E., Linker, S., Hedges, D. J., Batzer, M. A., & Deininger, P. L. (2015). Sequencing, identification and mapping of primed L1 elements (SIMPLE) reveals significant variation in full length L1 elements between individuals. BMC Genomics, 16(1). http://doi.org/10.1186/s12864-015-1374-y
Intended Use:
The LINE-1 Mobile Element NGS Assay kit is designed to profile L1H insertion sites in human DNA. This kit is for research use only.
Please read the entire user manual before proceeding with your experiment.
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