Box 1 Components

Description Concentration Quantity
Taq DNA Polymerase 50X 200 μl (2 vials)
Taq DNA Polymerase Buffer 10X 1,200 μl (2 vials)
dNTP Mix 50X (10 mM each) 200 μl
PCR-Grade Water NA 7,320 μl (4 vials)

Box 2 Components

Description
(Generic primer names: see Note below)
Concentration Quantity
Forward Primer 10 μM 150 μl
Reverse Primer 10 μM 150 μl
NFwd Primer 20X (10 μM) 240 μl
NRev Index Primer A 20X (10 μM) 20 μl
NRev Index Primer B 20X (10 μM) 20 μl
NRev Index Primer C 20X (10 μM) 20 μl
NRev Index Primer D 20X (10 μM) 20 μl
NRev Index Primer E 20X (10 μM) 20 μl
NRev Index Primer F 20X (10 μM) 20 μl
NRev Index Primer G 20X (10 μM) 20 μl
NRev Index Primer H 20X (10 μM) 20 μl
NRev Index Primer I 20X (10 μM) 20 μl
NRev Index Primer J 20X (10 μM) 20 μl
NRev Index Primer K 20X (10 μM) 20 μl
NRev Index Primer L 20X (10 μM) 20 μl
Seq NGS Primer* 100 μM 40 μl
Index NGS Primer 100 μM 40 μl

* In the NGS Prep Kits for CRISPR-Barcode and Dual-sgRNA Libraries, two unique Seq NGS Primers are included and are required for sequencing.

Note: The generic names for each of the primers are listed above for reference. However, each version of the NGS Prep Kit has primers specific for the vector of the target library. These specific primers names also include a suffix to indicate the vector or library they target (e.g., Forward-G, Forward-12, NRev-16, etc.). This Manual uses the generic primer name when, for example, it is describing which one to include in a reaction. For each generic primer reference, you should use the appropriate fully named specific primer included in your kit.

Storage Conditions:

Store kits and all components at -20°C until ready for use.

Last modified: Sep 10, 2019

Need more help with this?
Don’t hesitate to contact us here.

Thanks for your feedback.