Possible reason #1:

Incorrect First or Second Round PCR Primers or Sequencing Primer used.

Solution:

Ensure that you use the correct First and Second Round PCR Primers and Cellecta Sequencing Primer for your library. Check the Library Cassette Diagram provided with the kit.


Possible reason #2:

Incorrect NGS reagent kit used.

Solution:

Ensure that you or your NGS Core Facility uses the proper Illumina NGS reagent kit. For details, please check the kit descriptions in the Additional Materials Required section.

Last modified: Jan 10, 2019

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