Possible reason #1:
Incorrect First or Second Round PCR Primers or Sequencing Primer used.
Ensure that you use the correct First and Second Round PCR Primers and Cellecta Sequencing Primer for your library. Check the Library Cassette Diagram provided with the kit.
Possible reason #2:
Incorrect NGS reagent kit used.
Ensure that you or your NGS Core Facility uses the proper Illumina NGS reagent kit. For details, please check the kit descriptions in the Additional Materials Required section.
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