The PromoterTest™ Assay Custom Kit enables you to choose from a range of promoter constructs to easily identify the promoter that is optimally suited to express a cDNA of interest in your mammalian cell system. Each lentiviral construct is pre-packaged as ready-to-transduce lentiviral particles that can be transduced into your target cells. The lentivector carries the same green fluorescent protein (GFP) under the control of a different promoter. The intensity of the resulting fluorescence, as measured by FACS, correlates with the expression level of GFP. Higher GFP intensity indicates stronger promoter activity.
With this customizable PromoterTest™ Assay, you can measure the relative expression level between multiple promoters by assessing promoter strength (Fig.1) and sustained long-term expression (Fig.2) to find an optimal promoter that meets your expression requirements.
References and Product Citations for all Cellecta products can be found on the Cellecta website: https://www.cellecta.com/resources/publications/.
Please read the entire user manual before proceeding with your experiment. Also, please note that when working with pseudoviral particles, you should follow the recommended guidelines for working with Biosafety Level 2 (BSL-2) materials.
The purchase of all Cellecta Products is covered by Cellecta’s standard Terms and Conditions of Sale as described on Cellecta’s website, and selected Products containing particular technology or having certain features are also subject to restrictions on use as outlined in the Label License section of our website. Please review these Terms and Label License Restrictions before opening and using your Product and, if you are not able to abide by the restriction, contact Cellecta to return the item to Cellecta for a full refund.
Need more help with this?