• QIAamp DNA Micro Kit (QIAGEN, Cat.# 56304) — for <1M cells
  • DNeasy Blood and Tissue Kit (QIAGEN, Cat.# 69504) — for 1M-10M cells
  • Falcon® 15ml PP Screw-Cap Centrifuge Tubes (12,000 RCF rated, P:CHCl3-resistant) (Corning, Cat.# 352196)
  • Buffer P1 (50mM Tris-HCl pH 8.0, 10mM EDTA) (QIAGEN, Cat.# 19051)
  • Sodium Dodecyl Sulfate (SDS), 10% solution (Thermo Fisher, Cat.# 24730020)
  • RNase A (QIAGEN, Cat.# 19101)
  • Sonicator, for shearing Genomic DNA
  • Proteinase K from Tritirachium album (Sigma-Aldrich, Cat.# P4850)
  • Phenol:Chloroform:Isoamyl Alcohol 25:24:1 pH 8.0 (Sigma-Aldrich, Cat.# P3803)
  • Sodium Acetate, 3M solution (Sigma-Aldrich, Cat.# 71196- 100ML)
  • Isopropanol (2-Propanol) (Sigma-Aldrich, Cat.# I9516-500ML)
  • Linear Acrylamide (5 mg/ml) (Thermo Fisher, Cat.# AM9520) — for <5M cells

Other than the specific reagents and instruments listed above, the protocols assume the user has access to standard materials (e.g., polypropylene tubes, pipette tips), equipment (table top centrifuges, pipettes, scales), and common reagents (e.g., TE buffer, ethanol) and buffers used in a typical life science laboratory.

Last modified: 4 July 2018

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